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Determination of Hydrazine and 1-Methylhydrazine in Blood Serum

Report Number: AMRL TDR 64-24
Author: Reynolds, Barbara A.
Author: Thomas, Anthony A.
Corporate Author: Biomedical Laboratory
Laboratory: Biomedical Laboratory
Date of Publication: 1964-04
Pages: 16
Contract: Laboratory Research - No Contract
Project: 6302
Task: 630202
AD Number: AD0441231

A simple procedure is described for measuring microgram amounts of hydrazine in the blood serum of rats. The procedure, with a minor modification, can be used for measuring microgram amounts of 1-methylhydrazine. The report presents calibration ranges of 0.5-5.0 micrograms/ml and 0.5-10.0 micrograms/ml of hydrazine and 1-methylhydrazine, respectively. Data are presented on the dose-blood-level relationship of hydrazine and 1-methylhydrazine in rats following intraperitoneal injection. Minimum detectable dose levels were 0.6 mg/kg and 3.0 mg/kg of hydrazine and 1-methylhydrazine, respectively.

RAF Centre of Aviation Medicine
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