Contrails
The goal of the Contrails project is to help preserve and disseminate the technical record of 20th century aerospace research, highlighting in particular the research endeavors of the Illinois Tech community.

Effects of Cold Stress on Cellular Structure and Function

Report Number: AMRL TR 66-30
Author(s): Ralph Buchsbaum; Monte Buchsbaum; Thomas Linsenmayer
Corporate Author: University of Pittsburgh
Laboratory: Aerospace Medical Research Laboratories
Date of Publication: 1966-05
Pages: 15
Contract: AF 33(615)-1076
Project: 7164
Task: 716409
AD Number: AD0638327

Abstract:
The purpose of this investigation was to ascertain more precisely the limits of tolerance of cells in vitro to cold, as a basis for investigations on the cellular mechanisms affected. Mouse kidney cells were maintained in a perfusion chamber arranged for cooling and warming at various rates. Normal tissue culture media were used. Nearly all cells exposed to -0.3 C for 10 minutes, then warmed to 37 C, lived. Nearly all cells exposed to -1 C or lower for 10 minutes, and warmed to 37 C, died. The critical lethal cold shock seems to be between -0.3 and -0.7 C. Cold shock seems to produce little obvious change in cells, but mitochondrial breaks and swelling are obivous in electron micrographs. Also, nuclear ground substance appeared more granular than normal. One hypothesis was that microcrystallization probably occurs in certain organelles, particularly the mitochondria because these organelles are in constant motion. Similar results were obtained in experiments with human neutrophiles.

Download this report

(PDF, 0.8 MB, 15 pages)

Other options for obtaining this report

Via the Defense Technical Information Center (DTIC):
A record for this report, and possibly a full-image download of the report, exists at DTIC

Via National Technical Information Service:
This report may have been digitized and made freely available for public download, or made publicly available for purchase by the National Technical Information Service through their online database, the National Technical Reports Library

Indications of Public Availability
No digital image of an index entry indicating public availability is currently available
There has been no verification of an indication of public availability from an inside cover statement